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Course: Resonance Spectroscopies

Department/Abbreviation: KBF/RESP

Year: 2021

Guarantee: 'doc. RNDr. Pavel Pospíšil, Ph.D.'

Annotation: Introduction to theory, spectra, methods, experimental instrumentation and biological application of nuclear magnetic resonance, electron paramagnetic resonance and Mössbauer spectroscopy.

Course review:
1. Introduction in resonance spectroscopy. Principle of resonance spectroscopy. Quantum-mechanistic characterization of atomic nucleus and electrons. 2. Nuclear magnetic resonance (NMR). NMR theory, static, time-dependent a pulsed magnetic field, classical theory (precession, nutation, relaxation process), quantum theory (splitting of energy levels, Zeeman effect), phenomenological theory (magnetization, response function, continuous and pulsed solution of Bloch equations, free induction decay, spin echo). NMR spectra, number, intensity, position and splitting of NMR signal. NMR methods, one-dimensional NMR, 1H-NMR (NOED, ID NMR), 13C-NMR (broad band, off resonance, spin-echo), multi-dimensional NMR, homonuclear (COSY, MQF-COSY, NOESY), heteronuclear (HETCOR, COLOC, HSQC, HMQC, HMBC), NMR imaging. Experimental setup (magnet, frequency generator, detector), continuous and pulsed NMR spectrometer. Application of NMR in biology. 3. Electron paramagnetic resonance (EPR). EPR theory, splitting of energy levels, Zeeman effect, free radical, transitions metal, spin-orbital interaction (g-factor, anisotropy), spin-spin interaction (electron-nucleus), spin-spin interaction (electron-electron), comparison of EPR a NMR. EPR spectra, number, intensity, position and splitting of EPR signal. EPR methods, continuous EPR (EPR spin-trapping, EPR labeling, cw ENDOR/ELDOR), pulsed EPR (FT-EPR, pulsed ENDOR/ELDOR, ESEEM). Experimental setup (magnet, klystrons, resonator, cryogenic technique). Application of EPR v biology. 4. Mössbauer spectroscopy. Theory, Mössbauer effect, emission and absorption of gamma ray, nucleus free to recoil or bound in crystal lattice. Mössbauer spectra, hyperfine interaction (monopole, quadrupole, magnetic dipole), qualitative and quantitative analysis. Methods of Mössbauer spectroscopy. Experimental setup, Mössbauer spectrometer. Application of Mössbauer spectroscopy in biology.