PGSFM

Course: Fluorescence Methods in Photosynthesis

Department/Abbreviation: KBF/PGSFM

Year: 2021

Guarantee: 'prof. RNDr. Dušan Lazár, Ph.D.'

Annotation:

• Types of luminescence, fluorescence, experimental methods of measurements of particular parameters of chlorophyll fluorescence

Course review:

• Types of luminescence, Jablonski diagram of organic molecules
• Quantum-chemical methods of calculation of chlorophyll molecule and interpretation of absorption spectra
• Fluorescence, phosphorescence and delayed light emissions of chlorophylls in the model systems
• Fluorescence of chlorophyll a, b and carotenoids
• Parameters of in vivo fluorescence
• Emission and excitation spectra at different temperatures, their interpretation and usage
• Fluorescence re-absorption and its quantification
• Fluorescence transients (very fast, fast and slow fluorescence transients)
• QB-reducing/QB-non-reducing, alpha/beta and other types of photosystem II centers
• Fluorescence extinction - photochemical and non-photochemical extinction
• Parameters determined from fluorescence induction curves and from induction of extinction and their interpretation
• Fluorescence temperature curve, its phase, parameters and their interpretations
• Fluorescence kinetics in ps- and ns-time range, lifetime
• Polarization and coherence of chlorophyll fluorescence
• Experimental methods of measurements of particular parameters of chlorophyll fluorescence