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Course: Molecular Biophysics

Department/Abbreviation: KBF/MOLBI

Year: 2021

Guarantee: 'prof. RNDr. Jana Kašpárková, Ph.D.'

Annotation: Cells and cellular organells.Biological membranes.Membrane transport,receptors.Cellular dynamics.

Course review:
1. Origin and evolution of life. 2. Cell, cellular organelle. 3. Biological membranes - basic function. Type of cellular membranes. Basic components of BM and their formation. 4. Structure of biological membranes. Levels of studies of BM. Development of opinions on BM structure. Basic characteristics of BM 5. Physical properties of biological membranes. Organization of membrane lipids. Phase transitions. Mobility of membrane components. 6. Membrane transport I. Basis characteristics of major transport mechanisms. Diffusion. Transfer of ions across membrane. Mediated diffusion (carriers, channels). 7. Membrane transport II. Active transport. F- a V-ATPases. P-ATPases. Secondary active transport. Transportation of water. 8. Reception and processing of information by membranes. Physical signals. External chemical signals. Molecules of cell surface. Cellular recognition. 9. Receptors, receptor response I. Sorting of surface receptors. Receptors with integral and separated protein-kinase activity. Mechanism of receptor response. Metabolic pathways via cAMP and InsP3/DAG. 10. Receptors, receptor response II. Interaction, regulation and termination of receptor response. Endocytosis mediated by receptors. Cellular adhesion. 11. Cell dynamics: Cytoskeleton. Cellular contacts. Molecular motors. Active motion. 12. Antigens, immunogens and antibodies, immunogenicity. Epitopes, haptens. Immunoglobulins - structure, types. 13. Immune system. Lymphoid organs. Immune response of cells. Differentiation of B- a T-lymphocytes. Inborn and required immunity. 14. Immune response. Kinetics of immune response. Mechanisms of removal of antigens. Regulation of immune response. Cytokines (interleukins, tumor necrosis factor, interferons). Complement system. 15. Fundamental immunological methods. Immunoprecipitation curve. Immunodiffusion (simple, dual). Electrophoresis, immunoelectrophoresis. Electroimmunodiffusion. Chromatography. Nephelometry. Binding tests - RIA, EMIT, ELISA.