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Course: Microscopy and X-ray Structural Analysis

Department/Abbreviation: KBF/MKRSA

Year: 2021

Guarantee: 'RNDr. Roman Kouřil, Ph.D.'

Annotation: Microscopy and preparation of samples . Theory of structure of crystalline materials, experimental methods of X-ray structural analysis.

Course review:
- Light microscopy - theory of visual display in the light microscope, magnification, resolution efficiency, constructive parts of the light microscope, displaying methods (bright and dark field, phase contrast, Nomarski differential interference contrast, Hoffman modulation contrast, UV and IR spectroscopy, fluorescent microscopy), preparation of samples for the light microscope, confocal laser scanning and tandem microscopy, near-field microscopy. PALM, STORM, ..superresolution - Electron microcopy - principles of electron microscopy, interaction of electrons with a solid, basics of electron optics, resolution efficiency and depth of sharpness of the electron microscope, TEM, SEM, working regimes of the electron microscope, environmental electron microscope, preparation of samples for the electron microscope, fixation, dehydration, drying and sputtering, ultramicrotomy, methods of frozen etching and rapture, analytical electron microscopy (WDS, EDS). - Scanning probe microscopy - overview of methods of scanning probe microscopy, scanning tunneling microscopy, atomic force microscopy (AFM), LFM, MFM - X-ray structural analysis - theory of structure of crystalline materials, transformation of symmetry, lattice, elementary cells, Miller indices, elements and operations of symmetry, Bravais elementary cells, outward shape of a crystal and defects in crystals, Quasicrystals and amorphous materials, theory of percolation, physics of X-rays, theory of diffraction (Bragg equation, reciprocal lattice, Laue conditions, intensity of diffraction maxima), experimental methods of X-ray structural analysis, sources and detectors of X-rays, Debye-Scherrer method, textures, Laue method, method of crystal rotation, precession Weissenberg method, scattering at small angles.