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Course: Experimental Methods in Plant Physiology

Department/Abbreviation: KBF/EMFR

Year: 2021

Guarantee: 'doc. RNDr. Martina Špundová, Ph.D.'

Annotation: Knowledge of principles and use of experimental methods in plant physiology.

Course review:
1. Parameters of water status (RWC, water potential and its components). Estimation of water content from reflectance spectra. Measurement of soil water status. Transpiration and water transport (gravimetry, potometry, gasometry, rate of xylem transport. Measurement of ion leakage through membranes. 2. Photosynthetically active radiation (PAR): sources, spectra, units, measurement. PAR in canopy, LAI. Optical phenomena in leaf (reflectance, transmittance). Analytical and non-destructive measurement of pigment content in plants. Chloroplast movement. Optical indexes used in plant physiology. 3. Plant description. Structure of photosynthetic apparatus of higher plants (leaf, chloroplast, thylakoid membranes, pigment-protein complexes). 4. Chlorophyll fluorescence, fluorescence induction (definition, properties, fluorimeters, measurement, parameters). Remote-sensing. Phenotyping. 5. Photosynthetic oxygen evolution. Rate of CO2 assimilation (IRGA, gasometric systems, parameters). Light- and CO2-curves of photosynthesis. 6. Plant senescence. Definition, importance, types of senescence. Senescence-induced changes. Methods in senescence studies. 7. Plant nanotechnology. Definition and properties of nanomaterials. Synthesis of nanoparticles. Methods of detection and characterization of nanoparticles. Nanotechnology in agriculture. Interaction of nanoparticles and plants. 8. Introduction to plant electrophysiology. Electrochemical potential. Nernst´s equation. Resting membrane potential, action and variation potential. Measurement of electric signals in plants: surface and intracellular methods, aphid-method, patch-clamp.