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Course: Practicum of basic laboratory methods

Department/Abbreviation: KBF/PZLM

Year: 2020

Guarantee: 'Mgr. Jitka Prachařová, Ph.D.'

Annotation: The aim of this course is to acquaint students with basic methods commonly used in biophysical and biochemical laboratories.

Course review:
1. Principles of work in the chemical laboratory, chemical utensils, basic laboratory techniques (weighing, measuring volume, pipetting) 2. Preparation of solutions of the exact composition 3. Centrifugation 4. Conductometry, pH 5. Absorption spectrophotometry 6. Isolation of genomic DNA 7. DNA analysis using agarose gel electrophoresis 8. Preparation of protein extracts and determination of protein concentration in the cell lysate 9. Turbidimetry