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PGSFM


Course: Fluorescence Methods in Photosynthesis

Department/Abbreviation: KBF/PGSFM

Year: 2020

Guarantee: 'doc. RNDr. Dušan Lazár, Ph.D.'

Annotation:

  • Types of luminescence, fluorescence, experimental methods of measurements of particular parameters of chlorophyll fluorescence

Course review:

  • Types of luminescence, Jablonski diagram of organic molecules
  • Quantum-chemical methods of calculation of chlorophyll molecule and interpretation of absorption spectra
  • Fluorescence, phosphorescence and delayed light emissions of chlorophylls in the model systems
  • Fluorescence of chlorophyll a, b and carotenoids
  • Parameters of in vivo fluorescence
  • Emission and excitation spectra at different temperatures, their interpretation and usage
  • Fluorescence re-absorption and its quantification
  • Fluorescence transients (very fast, fast and slow fluorescence transients)
  • QB-reducing/QB-non-reducing, alpha/beta and other types of photosystem II centers
  • Fluorescence extinction - photochemical and non-photochemical extinction
  • Parameters determined from fluorescence induction curves and from induction of extinction and their interpretation
  • Fluorescence temperature curve, its phase, parameters and their interpretations
  • Fluorescence kinetics in ps- and ns-time range, lifetime
  • Polarization and coherence of chlorophyll fluorescence
  • Experimental methods of measurements of particular parameters of chlorophyll fluorescence