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PGSEB
Course: Experimental Methods od Biophysics
Department/Abbreviation: KBF/PGSEB
Year: 2020
Guarantee: 'prof. RNDr. Petr Ilík, Ph.D.'
Annotation: Basic knowledge in experimental methods of biophysics.
Course review:
Native and denaturation electrophoresis of proteins and pigment-protein complexes
Gel-scanners and imaging
HPLC of photosynthetic pigments
EPR, electron spin echo, optically detected magnetic resonance, ENDOR
Linear and circular dichroism
FTIR and resonance Raman spectroscopy
Photoacoustic spectroscopy
Gasometric methods
Kinetics of luminescence, thermoluminescence and chemiluminescence.
AFM, STM and near field microscopy
Optical scissors, micromanipulation with molecules
Fluorescence microscopy and imaging methods
Experimental methods for examination and analysis of voice: Laryngoscopic methods (laryngoscopy, laryngostroboscopy, high-speed videolaryngoscopy, videokymography), physiologic methods (electroglottography, photoglottography, pneumography, pneumotachography, measurements of subglottal pressure), acoustic methods (microphones and recording, acoustic spectrography, voice range profile, MDVP, DSI). Measurement of vocal load (voice dosimetry, time dose, cycle dose, distance dose, radiated energy dose, dissipated energy dose, the use of accelerometers for voice measurement).