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PGIB


Course: Interactions of Biomolecules

Department/Abbreviation: KBF/PGIB

Year: 2020

Guarantee: 'doc. RNDr. Martin Kubala, Ph.D.'

Annotation:

Course review:
Bonding types, ion interactions, dipole ion interactions, dipole-induced ion interactions, van der Waals interactions, dipole-dipole interactions, dipole-induced dipole interactions, induced dipole interactions, covalent linkages, molecular orbital theory, hydrogen bonds. Proteins and nucleic acids, structure-stabilizing interactions, hydrogen bonds, salt bridges, hydrophobic interactions, environmental influences (pH, ionic strength). Reactive groups of proteins and nucleic acids, their covalent interactions, quantitative characterization of covalent interactions. Non-covalent interactions of proteins and nucleic acids, quantitative characterization of non-covalent interactions, binding constants, models with multiple binding sites binding to small and large DNA grooves, intercalations. Structural motifs of non-covalent interactions, binding to small and large DNA grooves, intercalations, binding pockets. Design of spectroscopic experiment for the investigation of molecular interactions, direct experiment, competitive experiment, labeling of biomolecules and ligands, evaluation of the experiment. Non-equilibrium interactions, reaction kinetics, mechanisms of inhibition of enzyme reactions, experimental methods of kinetic monitoring on different time scales. Modeling of molecular interactions, molecular docking, quantum-chemical methods.